We have developed a novel, fast, and low cost protocol for isolating extracellular RNA and proteins from urine that gives a higher yield than current commercial kits. Furthermore, we have written a novel bioinformatics protocol that mines high-throughput sequencing data for known and novel disease-linked miRNAs. We are using our extracellular vesicle isolation protocol and our bioinformatics protocol to find biomarkers for prostate and bladder cancer. You can buy our kits here.
Ymatrix Concentrates EVs in SEC Fractions
Ymatrix is perfect for the rapid, low-cost, concentration of EV-associated nucleic acid and protein from dilute SEC fractions.
SEC is a very popular method for the rapid isolation of EVs from biofluids. However, although SEC columns effectively separate EVs from free protein they also greatly dilute the EV fraction. Thus, expensive or time-consuming post-SEC concentration is often necessary. Ymatrix can rapidly concentrate EV-associated biomolecules from SEC fractions in less than 20 minutes and at nearly half the cost of centrifugal filters.
Urine ExtraCellular Biomarker Discovery Service
Now Ymir Genomics is teaming up with the highly regarded contract research organization, Cambridge Biolabs (CBL), to offer a unique service to researchers in the urine biomarker discovery field.
You simply send us urine samples from your rat, mouse, or human studies. We then can isolate extracellular vesicles preps, extracellular protein preps suitable for proteomics, or extracellular RNA preps suitable for transcriptomics. These preps can be sent back to you or given to CBL for qPCR studies for specific RNA species or microRNA array studies.